Trypanosoma rangeli sialidase lacks trans-sialidase activity
نویسندگان
چکیده
منابع مشابه
Trypanosoma rangeli sialidase lacks trans-sialidase activity.
Extracts and tissue culture supernatants of axenic forms of T. rangeli were assayed for the presence of sialidase and trans-sialidase activities. Using sialyl(alpha 2-3)lactose, sialyl(alpha 2-6)lactose, poly(alpha 2-8)N-acetylneuraminic acid, fetuin and 4-methylumbelliferyl-N-acetylneuraminic acid as sialic acid donors, and lactose as a sialic acid acceptor, no trans-sialidase activity was det...
متن کاملDesign of Trypanosoma rangeli sialidase mutants with improved trans-sialidase activity
A sialidase (EC 3.2.1.18) from the non-pathogenic Trypanosoma rangeli, TrSA, has been shown to exert trans-sialidase activity after mutation of five specific amino acids in the active site (M96V, A98P, S120Y, G249Y, Q284P) to form the so-called TrSA5mut enzyme. By computational and hypothesis driven approaches additional mutations enhancing the trans-sialidase activity have been suggested. In t...
متن کاملTrypanosoma rangeli sialidase: cloning, expression and similarity to T. cruzi trans-sialidase.
Sialidases are hydrolytic enzymes present from virus to higher eukaryotes, catalyzing the removal of sialic acid from glycoconjugates. Some protozoa Trypanosomatidae secrete high levels of sialidase into the medium. We have now purified the secreted sialidase from Trypanosoma rangeli. Its N-terminal sequence reveals 100% identity with the corresponding region of the trans-sialidase from T. cruz...
متن کاملRational Design of a New Trypanosoma rangeli Trans-Sialidase for Efficient Sialylation of Glycans
This paper reports rational engineering of Trypanosoma rangeli sialidase to develop an effective enzyme for a potentially important type of reactivity: production of sialylated prebiotic glycans. The Trypanosoma cruzi trans-sialidase and the homologous T. rangeli sialidase has previously been used to investigate the structural requirements for trans-sialidase activity. We observed that the T. c...
متن کاملTrypanosoma rangeli sialidase: lack of activity in stocks from Panama and other regions.
A total of 33 crude and cloned Trypanosoma rangeli stocks found as natural infections in human from Panama and other endemic areas of Central and South America were evaluated as producers of sialidase (SA) activity through the MU-NANA fluorescence test. Negative results were observed in 6 of the isolates: Panama (4), Honduras (1), and Brazil (1). In addition, an immunoblotting analysis confirm ...
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ژورنال
عنوان ژورنال: Molecular and Biochemical Parasitology
سال: 1993
ISSN: 0166-6851
DOI: 10.1016/0166-6851(93)90173-u